GlycoFlux™ Human AMY1A Overexpression HepG2 Cell Line (CAT#: GLJF-0925-JF612)

Description	GlycoFlux™ human AMY1A overexpression HepG2 cell line is a stable cell line engineered to produce AMY1A, a salivary amylase that hydrolyzes α-1,4-glucosidic bonds in starch and glycogen to initiate carbohydrate digestion. Multiple transcript variants encode the same protein. This system supports research into digestive physiology, enzyme regulation, and carbohydrate metabolism.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	AMY1A
Full Name	Amylase Alpha 1A
Alternative Name	AMY1
Location	1p21.1
Gene ID	276
Summary	Amylases are secreted enzymes that hydrolyze 1,4-alpha-glucosidic bonds in starch and glycogen, initiating carbohydrate digestion. Multiple amylase genes are highly expressed in salivary glands or the pancreas. This gene encodes a salivary amylase isoenzyme, with alternative splicing generating multiple transcript variants of the same protein.