GlycoFlux™ Human AMY1A Overexpression Raji Cell Line

GlycoFlux™ human AMY1A overexpression Raji cell line is a stable cell line engineered to overexpress AMY1A, a salivary α-amylase isoenzyme that hydrolyzes 1,4-α-glucosidic bonds in starch and glycogen, catalyzing the first step in dietary carbohydrate digestion. The human genome contains a cluster of amylase genes highly expressed in salivary glands or pancreas, and AMY1A encodes a salivary isoform. This model is useful for exploring extracellular amylase function in lymphoma cells, nutrient availability, and tumor-microenvironment interactions related to carbohydrate metabolism.

Overexpression Cell Lines

Human

Lymphoblast-like, suspension growth

1:2~1:4

Raji

Burkitt's lymphoma

B lymphocyte (B cell)

Mature B cell, widely used in EBV-related studies

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

qPCR, Sanger Sequencing

FBS & RPMI

Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

AMY1A

Amylase Alpha 1A

AMY1

1p21.1

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. Alternative splicing results in multiple transcript variants encoding the same protein.