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GlycoFlux™ MMLV-Human PPP1R16B Viral Particle

CAT#: GLJF-1025-JF477

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SPECIFIC INQUIRY
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Titer:
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Specifications Quality Control Target Information Related Products
Specifications
Description
GlycoFlux™ MMLV-PPP1R16B Viral Particle delivers PPP1R16B, a membrane-anchored PP1 adaptor (ankyrin repeats, PP1-binding domain, CAAX) repressed by TGF-β1; the vector establishes stable lines to study membrane-proximal PP1 signaling and TGF-β axis modulation.
Product Type
Recombinant MMLV Retrovirus
Target
PPP1R16B
Species
Human
Promotor
MMLV 5' LTR promoter
Packaging System
Retrovirus
Packaging Cell
HEK293T
Application
This MMLV vector is a common retroviral vector system that permanently integrates a gene into the host cell's genome, allowing for long-term, sustained expression. It is a popular tool for gene manipulation in dividing cells and for generating iPS cells in biomedical research.
Shipping
Shipped on dry ice with temperature held at or below -80°C.
Storage
Store at -80°C to maintain their titer and stability.
Handling Notes
Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.
Product Disclaimer
These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Quality Control
Titer Assay
qPCR
Mycoplasma Test
Negative
Sterility
Sterility testing confirmed no microbial contamination.
Transduction Evaluation
On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation
Creative Biolabs verifies all retroviral vectors by a PCR-based proviral integration check. In brief, cells are transduced with serial dilutions of the retroviral preparation and, after several days, genomic DNA is extracted. A predefined segment of the expected retroviral insert is then amplified by PCR to confirm correct identity and integration.
Target Information
Full Name
Protein Phosphatase 1 Regulatory Subunit 16B
Alternative Name
TIMAP; ANKRD4
Location
20q11.23
Gene ID
26051
Summary
The encoded protein features ankyrin repeats, a PP1-binding domain, and a CAAX box for membrane attachment. It is repressed by TGF-beta-1 and participates in PP1-mediated signaling.
For Research Use Only.
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