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GlycoFlux™ MMLV-PPP1R9A Viral Particle provides PPP1R9A (Neurabin-1), an imprinted PDZ/SAM scaffold that orchestrates PP1 targeting and actin remodeling; this vector establishes stable mammalian expression lines for cytoskeletal signaling and isoform-resolved studies.
Product Type
Recombinant MMLV Retrovirus
Target
PPP1R9A
Species
Human
Promotor
MMLV 5' LTR promoter
Packaging System
Retrovirus
Packaging Cell
HEK293T
Application
This MMLV vector is a common retroviral vector system that permanently integrates a gene into the host cell's genome, allowing for long-term, sustained expression. It is a popular tool for gene manipulation in dividing cells and for generating iPS cells in biomedical research.
Shipping
Shipped on dry ice with temperature held at or below -80°C.
Storage
Store at -80°C to maintain their titer and stability.
Handling Notes
Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.
Product Disclaimer
These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Quality Control
Titer Assay
qPCR
Mycoplasma Test
Negative
Sterility
Sterility testing confirmed no microbial contamination.
Transduction Evaluation
On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation
Creative Biolabs verifies all retroviral vectors by a PCR-based proviral integration check. In brief, cells are transduced with serial dilutions of the retroviral preparation and, after several days, genomic DNA is extracted. A predefined segment of the expected retroviral insert is then amplified by PCR to confirm correct identity and integration.
An imprinted PP1 regulatory subunit gene, maternally expressed in embryonic muscle, encoding a protein with PDZ and SAM domains. It regulates actin cytoskeleton remodeling and has several isoforms.