Specifications Quality Control Target Information Related Products

Specifications

Description

GlycoFlux™ MSCV-PGK1 Viral Particle enforces persistent PGK1 expression, coupling ATP generation at the 1,3-BPG → 3-PG step with secreted, angiostatin-generating cofactor activity. Supports tumor metabolism and angiogenesis studies; assess ATP yield, endothelial tube formation, and DNA polymerase-α cofactor interactions.

Product Type

Recombinant MSCV Retrovirus

Target

PGK1

Species

Human

Promotor

MSCV 5' LTR promoter

Packaging System

Retrovirus

Packaging Cell

HEK293T

Application

This MSCV vector is a common retroviral vector system used to achieve high-efficiency, stable gene delivery into target cells, such as hematopoietic stem cells. It ensures long-term, sustained expression by stably integrating the gene into the host cell's genome.

Shipping

Dispatched on dry ice with temperature held at or below -80°C.

Storage

Store at -80°C to preserve titer and stability.

Handling Notes

Store at -80°C and avoid repeated freeze-thaw cycles to preserve viral titer. For safety, handle materials in a biological safety cabinet while wearing appropriate PPE (lab coat, gloves, and eye protection).

Product Disclaimer

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

Quality Control

Titer Assay

qPCR

Mycoplasma Test

Negative

Sterility

Sterility testing confirmed no microbial contamination.

Transduction Evaluation

Creative Biolabs offers, upon request, in vitro and in vivo transduction assays to verify lentiviral targeting and to quantify transgene expression and functional output.

Insert Identity Confirmation

All Creative Biolabs retroviral vectors undergo PCR-based verification of proviral integration. Cells are transduced across a dilution series of the retroviral preparation; after several days, genomic DNA is isolated, and a defined region of the expected insert is PCR-amplified to confirm correct identity and integration.

Target Information

Full Name

Phosphoglycerate Kinase 1

Alternative Name

PGKA; MIG10; HEL-S-68p

Location

Xq21.1

Gene ID

5230

Summary

The encoded protein catalyzes the conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate in glycolysis and can act as a cofactor for polymerase alpha. It is also secreted by tumor cells to promote angiogenesis via angiostatin generation. Deficiency can cause hemolytic anemia and neurological symptoms. Pseudogenes are found on chromosomes 19, 21, and X.

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