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GlycoFlux™ MSCV-Human PHKA1 Viral Particle (CAT#: GLJF-1025-JF717)

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SPECIFIC INQUIRY
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Description GlycoFlux™ MSCV-PHKA1 Viral Particle delivers the skeletal-muscle α regulatory subunit (PHKA1) for durable control of phosphorylase kinase in glycogenolysis. Use to reconstruct muscle-specific regulation; measure PhK activity, phosphorylase a/b ratios, and calcium/calmodulin-dependent modulation.
Product Type Recombinant MSCV Retrovirus
Target PHKA1
Species Human
Promotor MSCV 5' LTR promoter
Packaging System Retrovirus
Packaging Cell HEK293T
Application This MSCV vector is a common retroviral vector system used to achieve high-efficiency, stable gene delivery into target cells, such as hematopoietic stem cells. It ensures long-term, sustained expression by stably integrating the gene into the host cell's genome.
Shipping Dispatched on dry ice with temperature held at or below -80°C.
Storage Store at -80°C to preserve titer and stability.
Handling Notes Store at -80°C and avoid repeated freeze-thaw cycles to preserve viral titer. For safety, handle materials in a biological safety cabinet while wearing appropriate PPE (lab coat, gloves, and eye protection).
Product Disclaimer These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Titer Assay qPCR
Mycoplasma Test Negative
Sterility Sterility testing confirmed no microbial contamination.
Transduction Evaluation Creative Biolabs offers, upon request, in vitro and in vivo transduction assays to verify lentiviral targeting and to quantify transgene expression and functional output.
Insert Identity Confirmation All Creative Biolabs retroviral vectors undergo PCR-based verification of proviral integration. Cells are transduced across a dilution series of the retroviral preparation; after several days, genomic DNA is isolated, and a defined region of the expected insert is PCR-amplified to confirm correct identity and integration.
Full Name Phosphorylase Kinase Regulatory Subunit Alpha 1
Alternative Name PHKA
Location Xq13.1
Gene ID 5255
Summary Phosphorylase kinase is composed of alpha, beta, gamma, and delta subunits. The alpha subunit in skeletal muscle is encoded by this gene. Mutations result in glycogen storage disease type 9D (X-linked muscle glycogenosis). Alternative splicing produces different isoforms, and a pseudogene exists on chromosome 1.
For Research Use Only.
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