GlycoSens™ Soil Neutral Protease (S-NPT) Assay Kit, Spectrophotometry

The GlycoSens™ Soil Neutral Protease (S-NPT) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of soil neutral protease. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Soil Series

S-NPT

Sample Lysis Buffer; Substrate; Detection Reagent; Standard

Soil, sediments, solid substrates

50 μL

Target soil neutral protease (S-NPT) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target S-NPT.

Within 2 hours

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Soil Neutral Protease

Soil Neutral Protease; S-NPT

As a pivotal biomolecule, soil neutral protease (S-NPT) plays a central role in complex physiological networks. Monitoring it helps in understanding the ecological balance and nutrient availability in soil environments. In addition, investigating its functional state is crucial for identifying novel biomarkers and therapeutic targets.

Essential for monitoring soil neutral protease (S-NPT) level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.

High Specificity; Stable Reaction; Broad Sample Compatibility

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.