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Quantitative Human ABCA4 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX500)

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1×48 T
1×96 T

Product Description The quantitative human ABCA4 (glycosylated) sandwich ELISA kit is designed to detect human ATP binding cassette transporter A4 (ABCA4) levels. ABCA4 is a protein crucial for vision, which resides in the retina's photoreceptor cells. This protein functions as an energy-dependent flippase, actively transporting N-retinylidene-PE, a vitamin A derivative, across the membrane. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.129 ng/mL, which can accurately detect low concentrations of ABCA4 in the sample.
Target ABCA4
N-Glycosylation Site 98, 415, 444, 504, 1469, 1529, 1588, 1662
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.129 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name ATP binding cassette transporter A4
Alternate Names ABCA4; ATP binding cassette transporter A4; ABCR; ARMD2; RMP; RP19; STGD; Stargardt disease protein; Retinal-specific ATP-binding cassette transporter
Uniprot No. P78363
Application The quantitative human ABCA4 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated ABCA4 levels. The kit is a valuable tool for researchers investigating the role of ABCA4 in retinal diseases. It can be utilized in studies focused on understanding disease mechanisms, evaluating potential therapeutic interventions, and monitoring ABCA4 protein levels in various experimental models.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-105%;
Plasma sample: n=4, 95-110%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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