Quantitative Human ABCA4 (Glycosylated) Sandwich ELISA Kit

The quantitative human ABCA4 (glycosylated) sandwich ELISA kit is designed to detect human ATP binding cassette transporter A4 (ABCA4) levels. ABCA4 is a protein crucial for vision, which resides in the retina's photoreceptor cells. This protein functions as an energy-dependent flippase, actively transporting N-retinylidene-PE, a vitamin A derivative, across the membrane. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.129 ng/mL, which can accurately detect low concentrations of ABCA4 in the sample.

ABCA4

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.129 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-15 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

ATP binding cassette transporter A4

ABCA4; ATP binding cassette transporter A4; ABCR; ARMD2; RMP; RP19; STGD; Stargardt disease protein; Retinal-specific ATP-binding cassette transporter

98, 415, 444, 504, 1469, 1529, 1588, 1662

The quantitative human ABCA4 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated ABCA4 levels. The kit is a valuable tool for researchers investigating the role of ABCA4 in retinal diseases. It can be utilized in studies focused on understanding disease mechanisms, evaluating potential therapeutic interventions, and monitoring ABCA4 protein levels in various experimental models.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 90-105%;
Plasma sample: n=4, 95-110%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.