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Quantitative Human ACR (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX217

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Specifications
Product Description
The quantitative human ACR (glycosylated) sandwich ELISA kit is designed to detect human acrosin (ACR) levels. ACR is a serine protease located in the acrosome of sperm, crucial for penetrating the zona pellucida of the egg during fertilization. The kit is suitable for various biological samples such as serum. Its sensitivity is 0.081 ng/mL, which can accurately detect low concentrations of ACR in the sample.
Target
ACR
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Serum
Sample Volume
100 μL
Sensitivity
0.081 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Acrosin
Alternate Names
ACR; Acrosin; ACRS
Uniprot No.
N-Glycosylation Site
22, 210
Application Notes
Application
The quantitative human ACR (glycosylated) sandwich ELISA kit is valuable in research investigating male infertility, sperm function, and fertilization processes. Researchers utilize this ELISA to quantify ACR levels, providing insights into sperm quality and potential diagnostic applications related to male reproductive health.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 95-110%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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