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Quantitative Human ANO2 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX837

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Specifications
Product Description
The quantitative human ANO2 (glycosylated) sandwich ELISA kit is designed to detect human anoctamin 2 (ANO2) levels. ANO2 is a calcium-activated chloride channel that is a transmembrane protein expressed in various cell types. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 0.134 ng/mL, which can accurately detect low concentrations of ANO2 in the sample.
Target
ANO2
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Serum, plasma
Sample Volume
100 μL
Sensitivity
0.134 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Anoctamin 2
Alternate Names
ANO2; Anoctamin 2; Transmembrane protein 16B; TMEM16B
Uniprot No.
N-Glycosylation Site
422, 841, 849, 856
Application Notes
Application
The quantitative human ANO2 (glycosylated) sandwich ELISA kit is used to measure ANO2 levels in biological samples. This kit is applicable in studies examining ion channel functions and sensory systems. It is also relevant to studies investigating the role of ANO2 in smooth muscle physiology and other cellular processes.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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