Quantitative Human ASAH1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX570)

Name: Quantitative Human ASAH1 (Glycosylated) Sandwich ELISA Kit
SKU: GLJF-0525-HX570
Rating: 5 (1 reviews)

Datasheet

Size	Qty	Add To Basket
1×48 T
1×96 T

Product Description	The quantitative human ASAH1 (glycosylated) sandwich ELISA kit is designed to detect human N-Acylsphingosine amidohydrolase 1 (ASAH1) levels. ASAH1 is an enzyme that breaks down ceramide into sphingosine and fatty acids. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.935 ng/mL, which can accurately detect low concentrations of ASAH1 in the sample.
Target	ASAH1
N-Glycosylation Site	173, 195, 259, 286, 342, 348
Sample Types	Tissue homogenates, cell lysates
Sample Volume	100 μL
Sensitivity	0.935 ng/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	2.50 ng/mL-80 ng/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Species	Human
Full Name	N-Acylsphingosine amidohydrolase 1
Alternate Names	ASAH1; N-Acylsphingosine amidohydrolase 1; ASAH; PHP; Acid ceramidase; Acylsphingosine deacylase
Uniprot No.	Q13510

Application	The quantitative human ASAH1 (glycosylated) sandwich ELISA kit is a valuable tool for research in biochemistry and cell biology, particularly in studies of lipid metabolism. Researchers can measure ASAH1 levels in cells and tissues. Applications include studies of cancer, inflammation, and neurodegeneration.
Kit Components	Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 80-100%; Plasma sample: n=4, 80-95%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.

For Research Use Only.

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