Quantitative Human ASAH1 (Glycosylated) Sandwich ELISA Kit

The quantitative human ASAH1 (glycosylated) sandwich ELISA kit is designed to detect human N-Acylsphingosine amidohydrolase 1 (ASAH1) levels. ASAH1 is an enzyme that breaks down ceramide into sphingosine and fatty acids. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.935 ng/mL, which can accurately detect low concentrations of ASAH1 in the sample.

ASAH1

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.935 ng/mL

Quantitative sandwich ELISA

2.50 ng/mL-80 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

N-Acylsphingosine amidohydrolase 1

ASAH1; N-Acylsphingosine amidohydrolase 1; ASAH; PHP; Acid ceramidase; Acylsphingosine deacylase

173, 195, 259, 286, 342, 348

The quantitative human ASAH1 (glycosylated) sandwich ELISA kit is a valuable tool for research in biochemistry and cell biology, particularly in studies of lipid metabolism. Researchers can measure ASAH1 levels in cells and tissues. Applications include studies of cancer, inflammation, and neurodegeneration.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.