Quantitative Human CHRN α1 (Glycosylated) Sandwich ELISA Kit

The quantitative human CHRN α1 (glycosylated) sandwich ELISA kit is designed to detect human cholinergic receptor, nicotinic α1 (CHRN α1) levels. CHRN α1 is a subunit of the nicotinic acetylcholine receptor (nAChR), a ligand-gated ion channel crucial for neuromuscular transmission. It plays a key role in muscle contraction and is targeted in autoimmune diseases like myasthenia gravis. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.501 ng/mL, which can accurately detect low concentrations of CHRN α1 in the sample.

CHRN α1

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.501 ng/mL

Quantitative sandwich ELISA

2.25 ng/mL-50 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Cholinergic receptor, nicotinic α1

CHRN α1; Cholinergic receptor, nicotinic α1; CHR-NA1; NAChRA1; ACHRD; CMS2A; SCCMS; Acetylcholine receptor subunit α

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The quantitative human CHRN α1 (glycosylated) sandwich ELISA kit is valuable in research investigating neuromuscular disorders, myasthenia gravis, and the effects of nicotinic receptor agonists or antagonists. Researchers utilize this ELISA to quantify CHRNα1 levels, providing insights into its role in neuromuscular function and related pathologies.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 90-105%;
Plasma sample: n=4, 85-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.