Quantitative Human CHRNα5 (Glycosylated) Sandwich ELISA Kit

The quantitative human CHRNα5 (glycosylated) sandwich ELISA kit is designed to detect human cholinergic receptor, nicotinic, α5 (CHRNα5) levels. CHRNα5 is a subunit of the nicotinic acetylcholine receptor. This receptor is a ligand-gated ion channel. CHRNα5 plays a role in neurotransmission. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.581 ng/mL, which can accurately detect low concentrations of CHRNα5 in the sample.

CHRNα5

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.581 ng/mL

Quantitative sandwich ELISA

2.5 ng/mL-45 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Cholinergic receptor, nicotinic, α5

CHRNα5; Cholinergic receptor, nicotinic, α5; CHRN-A5; NAChRA5; Neuronal acetylcholine receptor α5

155, 183, 229

The quantitative human CHRNα5 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated CHRNα5 levels. It is useful for studying the role of CHRNα5 in nicotine dependence.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.