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Quantitative Human CRHR2 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX562)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human CRHR2 (glycosylated) sandwich ELISA kit is designed to detect human corticotropin releasing hormone receptor 2 (CRHR2) levels. CRHR2 is a G protein-coupled receptor, which binds corticotropin-releasing hormone (CRH) and related peptides. CRHR2 is expressed in the brain and peripheral tissues, which mediates the effects of CRH on stress response, anxiety, and cardiovascular functions. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of CRHR2 in the sample.
Target CRHR2
N-Glycosylation Site 13, 41, 74, 86, 94
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.125 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Corticotropin releasing hormone receptor 2
Alternate Names CRHR2; Corticotropin releasing hormone receptor 2; CRFR2; CRF2; Corticotropin-releasing factor receptor 2
Uniprot No. Q13324
Application The quantitative human CRHR2 (glycosylated) sandwich ELISA kit is a valuable tool for neuroscience and endocrinology research, particularly for studying stress response and related disorders. Researchers can measure CRHR2 levels in tissues. Applications include studies of anxiety, depression, and stress-related conditions.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-105%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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