Quantitative Human CRHR2 (Glycosylated) Sandwich ELISA Kit

The quantitative human CRHR2 (glycosylated) sandwich ELISA kit is designed to detect human corticotropin releasing hormone receptor 2 (CRHR2) levels. CRHR2 is a G protein-coupled receptor, which binds corticotropin-releasing hormone (CRH) and related peptides. CRHR2 is expressed in the brain and peripheral tissues, which mediates the effects of CRH on stress response, anxiety, and cardiovascular functions. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of CRHR2 in the sample.

CRHR2

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.125 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-15 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Corticotropin releasing hormone receptor 2

CRHR2; Corticotropin releasing hormone receptor 2; CRFR2; CRF2; Corticotropin-releasing factor receptor 2

13, 41, 74, 86, 94

The quantitative human CRHR2 (glycosylated) sandwich ELISA kit is a valuable tool for neuroscience and endocrinology research, particularly for studying stress response and related disorders. Researchers can measure CRHR2 levels in tissues. Applications include studies of anxiety, depression, and stress-related conditions.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-105%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.