Quantitative Human CRYαB (Glycosylated) Sandwich ELISA Kit

The quantitative human CRYαB (glycosylated) sandwich ELISA kit is designed to detect human crystallin αB (CRYαB) levels. CRYαB is a small heat shock protein that functions as a molecular chaperone, playing a crucial role in maintaining cellular protein homeostasis and preventing protein aggregation. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.501 ng/mL, which can accurately detect low concentrations of CRYαB in the sample.

CRYαB

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.501 ng/mL

Quantitative sandwich ELISA

2 ng/mL-40 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Crystallin αB

CRYαB; Crystallin αB; CRY-AB; CRYA2; HSPB5; Heat shock protein β5

41, 170

The quantitative human CRYαB (glycosylated) sandwich ELISA kit is valuable in research investigating cataracts, neurodegenerative diseases, and cardiac pathologies, where CRYαB's chaperone activity is implicated. Researchers utilize this ELISA to quantify CRYαB levels, providing insights into its role in protein aggregation-related diseases and potential therapeutic targets.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-105%;
Plasma sample: n=4, 90-110%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.