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Quantitative Human CRYαB (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX153)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human CRYαB (glycosylated) sandwich ELISA kit is designed to detect human crystallin αB (CRYαB) levels. CRYαB is a small heat shock protein that functions as a molecular chaperone, playing a crucial role in maintaining cellular protein homeostasis and preventing protein aggregation. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.501 ng/mL, which can accurately detect low concentrations of CRYαB in the sample.
Target CRYαB
O-Glycosylation Site 41, 170
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.501 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 2 ng/mL-40 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Crystallin αB
Alternate Names CRYαB; Crystallin αB; CRY-AB; CRYA2; HSPB5; Heat shock protein β5
Uniprot No. P02511
Application The quantitative human CRYαB (glycosylated) sandwich ELISA kit is valuable in research investigating cataracts, neurodegenerative diseases, and cardiac pathologies, where CRYαB's chaperone activity is implicated. Researchers utilize this ELISA to quantify CRYαB levels, providing insights into its role in protein aggregation-related diseases and potential therapeutic targets.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-105%;
Plasma sample: n=4, 90-110%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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