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Quantitative Human CXCR4 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX491

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Specifications
Product Description
The quantitative human CXCR4 (glycosylated) sandwich ELISA kit is designed to detect human chemokine C-X-C-motif receptor 4 (CXCR4) levels. CXCR4 is a G protein-coupled receptor that binds chemokine CXCL12, which is expressed on various cell types, including immune cells and stem cells. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.089 ng/mL, which can accurately detect low concentrations of CXCR4 in the sample.
Target
CXCR4
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.089 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Chemokine C-X-C-motif receptor 4
Alternate Names
CXCR4; Chemokine C-X-C-motif receptor 4; CD184; HSY3RR; LAP3; NPY3R; Lipopolysaccharide-associated protein 3
Uniprot No.
N-Glycosylation Site
11, 176
O-Glycosylation Site
18
Application Notes
Application
The quantitative human CXCR4 (glycosylated) sandwich ELISA kit is designed to measure glycosylated CXCR4 levels in samples. This measurement is valuable in research related to immunology and stem cell biology. The kit is applied in studies of cell migration, which helps in understanding the role of CXCR4.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-110%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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