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Quantitative Human ECP (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0325-HX268

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Specifications
Product Description
The quantitative human ECP (glycosylated) sandwich ELISA kit is designed to detect human eosinophil cationic protein (ECP) levels. It is suitable for various biological samples such as tissue homogenates, serum, or plasma. Its sensitivity is 0.412 ng/mL, which can accurately detect low concentrations of ECP in the sample.
Target
ECP
Kit Components
Pre-coated 96-well ELISA plate; Lyophilized standard; Biotinylated ECP antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
50 μL-100 μL
Sensitivity
0.412 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1.65 ng/mL-100 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Eosinophil cationic protein
Alternate Names
Cytotoxic ribonuclease; Eosinophil cationic protein; Ribonuclease 3; Ribonuclease; RNase A family 3; RNase 3; RNASE3; RNS3; ECP
Uniprot No.
N-Glycosylation Site
84, 92, 119
Application Notes
Application
The quantitative human ECP (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of ECP, helping to understand its role in conditions such as allergic reactions and asthma.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-95%;
Plasma sample: n=4, 90-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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