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Quantitative Human FFAR2 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX51)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human FFAR2 (glycosylated) sandwich ELISA kit is designed to detect human free fatty acid receptor 2 (FFAR2) levels. FFAR2 is a G protein-coupled receptor that is activated by short-chain fatty acids, primarily acetate. It plays a crucial role in regulating inflammation, metabolism, and gut health. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.111 ng/mL, which can accurately detect low concentrations of FFAR2 in the sample.
Target FFAR2
N-Glycosylation Site 151, 167
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.111 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Free fatty acid receptor 2
Alternate Names FFAR2; Free fatty acid receptor 2; FFA2R; GPR43; GPCR43; G-protein coupled receptor 43
Uniprot No. O15552
Application The quantitative human FFAR2 (glycosylated) sandwich ELISA kit is utilizes a sandwich enzyme-linked immunosorbent assay technique, employing specific antibodies to capture and detect the target protein, enabling researchers to accurately measure FFAR2 levels for various research applications.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-105%;
Plasma sample: n=4, 80-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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