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Quantitative Human GM-CSF (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0325-HX223

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Specifications
Product Description
The quantitative human GM-CSF (glycosylated) sandwich ELISA kit is designed to detect human granulocyte-macrophage colony stimulating factor (GM-CSF) levels. It is suitable for various biological samples such as tissue homogenates, cell culture supernates, serum, or plasma. Its sensitivity is 3.899 pg/mL, which can accurately detect low concentrations of GM-CSF in the sample.
Target
CSF2
Kit Components
Pre-coated 96-well ELISA plate; Lyophilized standard; Biotinylated GM-CSF antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell culture supernates, serum, plasma
Sample Volume
50 μL-100 μL
Sensitivity
3.899 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
15.65 pg/mL-1000 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Colony-stimulating factor 2
Alternate Names
Colony-stimulating factor 2; Csf2; Granulocyte-macrophage colony-stimulating factor; GMCSF; Colony-stimulating factor; CSF2
Uniprot No.
N-Glycosylation Site
44, 54
O-Glycosylation Site
22, 24, 26, 27
Application Notes
Application
The quantitative human GM-CSF (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of GM-CSF, helping to understand its role in immunology.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 90-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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