Quantitative Human GM-CSF (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0325-HX223)

Product Description	The quantitative human GM-CSF (glycosylated) sandwich ELISA kit is designed to detect human granulocyte-macrophage colony stimulating factor (GM-CSF) levels. It is suitable for various biological samples such as tissue homogenates, cell culture supernates, serum, or plasma. Its sensitivity is 3.899 pg/mL, which can accurately detect low concentrations of GM-CSF in the sample.
Target	CSF2
N-Glycosylation Site	44, 54
O-Glycosylation Site	22, 24, 26, 27
Sample Types	Tissue homogenates, cell culture supernates, serum, plasma
Sample Volume	50 μL-100 μL
Sensitivity	3.899 pg/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	15.65 pg/mL-1000 pg/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Species	Human
Full Name	Colony-stimulating factor 2
Alternate Names	Colony-stimulating factor 2; Csf2; Granulocyte-macrophage colony-stimulating factor; GMCSF; Colony-stimulating factor; CSF2
Uniprot No.	P04141

Application	The quantitative human GM-CSF (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of GM-CSF, helping to understand its role in immunology.
Kit Components	Pre-coated 96-well ELISA plate; Lyophilized standard; Biotinylated GM-CSF antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 85-100%; Plasma sample: n=4, 90-110%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.