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Quantitative Human GZMM (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX447)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human GZMM (glycosylated) sandwich ELISA kit is designed to detect human granzyme M (GZMM) levels. GZMM is a serine protease enzyme found in cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. GZMM is released by these immune cells to induce target cell death. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 5.937 pg/mL, which can accurately detect low concentrations of GZMM in the sample.
Target GZMM
N-Glycosylation Site 177
Sample Types Tissue homogenates, serum, plasma
Sample Volume 100 μL
Sensitivity 5.937 pg/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 20 pg/mL-800 pg/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Granzyme M
Alternate Names GZMM; Granzyme M; LMET1; MET1; Met-1 serine protease; Natural killer cell granular protease
Uniprot No. P51124
Application The quantitative human GZMM (glycosylated) sandwich ELISA kit is used to measure glycosylated GZMM levels. This measurement is valuable in immunological research, particularly studies of cytotoxic T cells and natural killer cells. The kit can be applied in investigations of immune responses to viral infections, cancer, and other conditions where cell-mediated cytotoxicity is important.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-105%;
Plasma sample: n=4, 80-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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