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Quantitative Human GZMM (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX447

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Specifications
Product Description
The quantitative human GZMM (glycosylated) sandwich ELISA kit is designed to detect human granzyme M (GZMM) levels. GZMM is a serine protease enzyme found in cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. GZMM is released by these immune cells to induce target cell death. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 5.937 pg/mL, which can accurately detect low concentrations of GZMM in the sample.
Target
GZMM
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
5.937 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
20 pg/mL-800 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Granzyme M
Alternate Names
GZMM; Granzyme M; LMET1; MET1; Met-1 serine protease; Natural killer cell granular protease
Uniprot No.
N-Glycosylation Site
177
Application Notes
Application
The quantitative human GZMM (glycosylated) sandwich ELISA kit is used to measure glycosylated GZMM levels. This measurement is valuable in immunological research, particularly studies of cytotoxic T cells and natural killer cells. The kit can be applied in investigations of immune responses to viral infections, cancer, and other conditions where cell-mediated cytotoxicity is important.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-105%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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