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Quantitative Human LIPC (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX225)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human LIPC (glycosylated) sandwich ELISA kit is designed to detect human lipase, hepatic (LIPC) levels. LIPC is an enzyme involved in lipoprotein metabolism, particularly the hydrolysis of triglycerides and phospholipids in high-density lipoproteins (HDL). It plays a crucial role in regulating HDL cholesterol levels. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 37.274 pg/mL, which can accurately detect low concentrations of LIPC in the sample.
Target LIPC
N-Glycosylation Site 42, 78, 362, 397
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 37.274 pg/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 100 pg/mL-4000 pg/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Lipase
Alternate Names LIPC; Lipase; HTGL; Hepatic triacylglycerol lipase
Uniprot No. P11150
Application The quantitative human LIPC (glycosylated) sandwich ELISA kit is valuable in research investigating lipid metabolism disorders, cardiovascular diseases, and the role of HDL in atherosclerosis. Researchers utilize this ELISA to quantify LIPC levels, providing insights into its impact on lipoprotein profiles and potential therapeutic targets.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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