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Quantitative Human LIPC (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX225

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Specifications
Product Description
The quantitative human LIPC (glycosylated) sandwich ELISA kit is designed to detect human lipase, hepatic (LIPC) levels. LIPC is an enzyme involved in lipoprotein metabolism, particularly the hydrolysis of triglycerides and phospholipids in high-density lipoproteins (HDL). It plays a crucial role in regulating HDL cholesterol levels. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 37.274 pg/mL, which can accurately detect low concentrations of LIPC in the sample.
Target
LIPC
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
37.274 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
100 pg/mL-4000 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Lipase
Alternate Names
LIPC; Lipase; HTGL; Hepatic triacylglycerol lipase
Uniprot No.
N-Glycosylation Site
42, 78, 362, 397
Application Notes
Application
The quantitative human LIPC (glycosylated) sandwich ELISA kit is valuable in research investigating lipid metabolism disorders, cardiovascular diseases, and the role of HDL in atherosclerosis. Researchers utilize this ELISA to quantify LIPC levels, providing insights into its impact on lipoprotein profiles and potential therapeutic targets.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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