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Quantitative Human LPO (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX304

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Specifications
Product Description
The quantitative human LPO (glycosylated) sandwich ELISA kit is designed to detect human lactoperoxidase (LPO) levels. LPO is an enzyme secreted by mammary glands and other mucosal tissues. LPO catalyzes the peroxidation of various substrates, including thiocyanate and iodide, using hydrogen peroxide. LPO plays a crucial role in the innate immune system, it protects mucosal surfaces from infection. The kit is suitable for various biological samples such as biological fluids. Its sensitivity is 0.085 ng/mL, which can accurately detect low concentrations of LPO in the sample.
Target
LPO
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Biological fluids
Sample Volume
100 μL
Sensitivity
0.085 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Lactoperoxidase
Alternate Names
LPO; Lactoperoxidase; SPO; Salivary peroxidase
Uniprot No.
N-Glycosylation Site
106, 212, 322, 358
Application Notes
Application
The quantitative human LPO (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated LPO in biological samples, which is particularly relevant in studies of mucosal immunity.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 95-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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