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Quantitative Human ME20M (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX403

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The quantitative human ME20M (glycosylated) sandwich ELISA kit is designed to detect human melanoma associated ME20 (ME20M) levels. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.069 ng/mL, which can accurately detect low concentrations of ME20M in the sample.
Target
ME20M
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.069 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Melanoma associated ME20
Alternate Names
ME20M; Melanoma associated ME20; PMEL; Premelanosome protein
Uniprot No.
P40967
N-Glycosylation Site
81, 106, 111, 321, 568
Application Notes
Application
The quantitative human ME20M (glycosylated) sandwich ELISA kit is used to quantify the target protein in biological samples. This quantification could be relevant to research areas studying the protein's functions, its involvement in disease processes, or its response to therapeutic interventions.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 90-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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