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Quantitative Human MTNR1A (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX430

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Specifications
Product Description
The quantitative human MTNR1A (glycosylated) sandwich ELISA kit is designed to detect human melatonin receptor 1A (MTNR1A) levels. MTNR1A is a G protein-coupled receptor that binds melatonin. Melatonin is a hormone that regulates circadian rhythms. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.105 ng/mL, which can accurately detect low concentrations of MTNR1A in the sample.
Target
MTNR1A
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.105 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Melatonin receptor 1A
Alternate Names
MTNR1A; Melatonin receptor 1A; MT1
Uniprot No.
N-Glycosylation Site
4, 10
Application Notes
Application
The quantitative human MTNR1A (glycosylated) sandwich ELISA kit is designed to measure glycosylated MTNR1A levels in samples. This measurement is valuable in research related to sleep disorders and circadian rhythm disruption. The kit is applied in studies of conditions such as insomnia, which helps in understanding the role of MTNR1A.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 90-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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