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Quantitative Human NFE2L2 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX611)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human NFE2L2 (glycosylated) sandwich ELISA kit is designed to detect human nuclear factor, erythroid derived 2 like protein 2 (NFE2L2) levels. NFE2L2 is a transcription factor that plays a crucial role in cellular defense against oxidative stress. It regulates the expression of a battery of antioxidant and detoxifying genes. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.092 ng/mL, which can accurately detect low concentrations of NFE2L2 in the sample.
Target NFE2L2
N-Glycosylation Site 462, 472, 487, 499, 569, 574
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.092 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Nuclear factor, erythroid derived 2 like protein 2
Alternate Names NFE2L2; Nuclear factor, erythroid derived 2 like protein 2; NRF2; NFE2-related factor 2
Uniprot No. Q16236
Application The quantitative human NFE2L2 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated NFE2L2 levels of this transcription factor in biological samples. The kit is applicable in studies of oxidative stress, antioxidant defense mechanisms, and cellular protection. It is useful in fields such as toxicology and environmental health.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 95-110%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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