Quantitative Human NFE2L2 (Glycosylated) Sandwich ELISA Kit

The quantitative human NFE2L2 (glycosylated) sandwich ELISA kit is designed to detect human nuclear factor, erythroid derived 2 like protein 2 (NFE2L2) levels. NFE2L2 is a transcription factor that plays a crucial role in cellular defense against oxidative stress. It regulates the expression of a battery of antioxidant and detoxifying genes. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.092 ng/mL, which can accurately detect low concentrations of NFE2L2 in the sample.

NFE2L2

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.092 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Nuclear factor, erythroid derived 2 like protein 2

NFE2L2; Nuclear factor, erythroid derived 2 like protein 2; NRF2; NFE2-related factor 2

462, 472, 487, 499, 569, 574

The quantitative human NFE2L2 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated NFE2L2 levels of this transcription factor in biological samples. The kit is applicable in studies of oxidative stress, antioxidant defense mechanisms, and cellular protection. It is useful in fields such as toxicology and environmental health.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 95-110%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.