Quantitative Human OTOL1 (Glycosylated) Sandwich ELISA Kit

The quantitative human OTOL1 (glycosylated) sandwich ELISA kit is designed to detect human otolin 1 (OTOL1) levels. Otolin 1 is a secreted glycoprotein encoded by the OTOL1 gene that contains a C-terminal complement C1q-like globular domain and contains extensive post-translational modifications, such as hydroxylated proline and glycosylated lysine, and can form multimeric complexes. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of OTOL1 in the sample.

OTOL1

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates, serum, plasma

100 μL

0.125 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Otolin 1

OTOL1; Otolin 1; C1QTNF15; C1QTNF16

202, 381

178, 310

The quantitative human OTOL1 (glycosylated) sandwich ELISA kit is widely used in the field of biomedical research to assist in exploring the physiological and pathological mechanisms associated with OTOL1 glycosylated proteins. For example, it can be used as a detection tool in disease diagnosis, disease monitoring, and drug development to evaluate the effects of disease states or drugs on OTOL1 glycosylation levels.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 90-100%;
Plasma sample: n=4, 90-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.