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| Size | Qty | Add To Basket |
|---|---|---|
| 1×48 T | ||
| 1×96 T |
| Product Description | The quantitative human OTOL1 (glycosylated) sandwich ELISA kit is designed to detect human otolin 1 (OTOL1) levels. Otolin 1 is a secreted glycoprotein encoded by the OTOL1 gene that contains a C-terminal complement C1q-like globular domain and contains extensive post-translational modifications, such as hydroxylated proline and glycosylated lysine, and can form multimeric complexes. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of OTOL1 in the sample. |
| Target | OTOL1 |
| N-Glycosylation Site | 202, 381 |
| O-Glycosylation Site | 178, 310 |
| Sample Types | Tissue homogenates, cell lysates, serum, plasma |
| Sample Volume | 100 μL |
| Sensitivity | 0.125 ng/mL |
| Detection Principle | Quantitative sandwich ELISA |
| Detection Range | 1 ng/mL-10 ng/mL |
| Detection Time | 1 h-5 h |
| Detection Wavelength | 450 nm |
| Storage | Store at 2-8°C for long term storage. |
| Species | Human |
| Full Name | Otolin 1 |
| Alternate Names | OTOL1; Otolin 1; C1QTNF15; C1QTNF16 |
| Uniprot No. | A6NHN0 |
| Application | The quantitative human OTOL1 (glycosylated) sandwich ELISA kit is widely used in the field of biomedical research to assist in exploring the physiological and pathological mechanisms associated with OTOL1 glycosylated proteins. For example, it can be used as a detection tool in disease diagnosis, disease monitoring, and drug development to evaluate the effects of disease states or drugs on OTOL1 glycosylation levels. |
| Kit Components | Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution |
| Precision | Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%; |
| Recovery | Serum sample: n=5, 90-100%; Plasma sample: n=4, 90-100%; |
| Standard Curve | ![]() The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested. |