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Quantitative Human OTOL1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX2)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human OTOL1 (glycosylated) sandwich ELISA kit is designed to detect human otolin 1 (OTOL1) levels. Otolin 1 is a secreted glycoprotein encoded by the OTOL1 gene that contains a C-terminal complement C1q-like globular domain and contains extensive post-translational modifications, such as hydroxylated proline and glycosylated lysine, and can form multimeric complexes. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of OTOL1 in the sample.
Target OTOL1
N-Glycosylation Site 202, 381
O-Glycosylation Site 178, 310
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.125 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Otolin 1
Alternate Names OTOL1; Otolin 1; C1QTNF15; C1QTNF16
Uniprot No. A6NHN0
Application The quantitative human OTOL1 (glycosylated) sandwich ELISA kit is widely used in the field of biomedical research to assist in exploring the physiological and pathological mechanisms associated with OTOL1 glycosylated proteins. For example, it can be used as a detection tool in disease diagnosis, disease monitoring, and drug development to evaluate the effects of disease states or drugs on OTOL1 glycosylation levels.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-100%;
Plasma sample: n=4, 90-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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