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Quantitative Human PLB (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX643

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Specifications
Product Description
The quantitative human PLB (glycosylated) sandwich ELISA kit is designed to detect human phospholipase B (PLB) levels. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of PLB in the sample.
Target
PLB
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates
Sample Volume
100 μL
Sensitivity
0.125 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Phospholipase B
Alternate Names
PLB; Phospholipase B; PLB1; Lysophospholipase
Uniprot No.
N-Glycosylation Site
173, 240, 493, 529, 590, 690, 783, 797, 809, 1055, 1113, 1275, 1378
Application Notes
Application
The quantitative human PLB (glycosylated) sandwich ELISA kit is used to determine PLB concentrations in biological samples. It proves valuable in exploratory research investigating the protein's role in lipid metabolism and cellular signaling pathways. This kit finds application in fields such as biochemistry, cell biology, and potentially in studies related to diseases involving altered lipid metabolism.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-105%;
Plasma sample: n=4, 90-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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