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Quantitative Human PLB (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX643)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human PLB (glycosylated) sandwich ELISA kit is designed to detect human phospholipase B (PLB) levels. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of PLB in the sample.
Target PLB
N-Glycosylation Site 173, 240, 493, 529, 590, 690, 783, 797, 809, 1055, 1113, 1275, 1378
Sample Types Tissue homogenates
Sample Volume 100 μL
Sensitivity 0.125 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Phospholipase B
Alternate Names PLB; Phospholipase B; PLB1; Lysophospholipase
Uniprot No. Q6P1J6
Application The quantitative human PLB (glycosylated) sandwich ELISA kit is used to determine PLB concentrations in biological samples. It proves valuable in exploratory research investigating the protein's role in lipid metabolism and cellular signaling pathways. This kit finds application in fields such as biochemistry, cell biology, and potentially in studies related to diseases involving altered lipid metabolism.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-105%;
Plasma sample: n=4, 90-105%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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