Quantitative Human PLOD3 (Glycosylated) Sandwich ELISA Kit

The quantitative human PLOD3 (glycosylated) sandwich ELISA kit is designed to detect human procollagen lysine-2-oxoglutarate-5-dioxygenase 3 (PLOD3) levels. PLOD3 is an enzyme that hydroxylates lysine residues in collagen, a critical step for collagen crosslinking and the stability of connective tissues. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.136 ng/mL, which can accurately detect low concentrations of PLOD3 in the sample.

PLOD3

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.136 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-15 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Procollagen lysine-2-oxoglutarate-5-dioxygenase 3

PLOD3; Procollagen lysine-2-oxoglutarate-5-dioxygenase 3; LH3; Lysyl hydroxylase 3

63, 548

The quantitative human PLOD3 (glycosylated) sandwich ELISA kit is designed to measure the concentration of glycosylated PLOD3 in biological samples. This is valuable for research investigating collagen-related disorders, such as fibrosis, skeletal abnormalities, and cancer. The quantitative data obtained from this kit allows for precise analysis of PLOD3 levels, aiding in understanding its role in collagen biosynthesis and disease pathogenesis.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-100%;
Plasma sample: n=4, 85-110%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.