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Quantitative Human PLOD3 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX74)

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1×48 T
1×96 T

Product Description The quantitative human PLOD3 (glycosylated) sandwich ELISA kit is designed to detect human procollagen lysine-2-oxoglutarate-5-dioxygenase 3 (PLOD3) levels. PLOD3 is an enzyme that hydroxylates lysine residues in collagen, a critical step for collagen crosslinking and the stability of connective tissues. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.136 ng/mL, which can accurately detect low concentrations of PLOD3 in the sample.
Target PLOD3
N-Glycosylation Site 63, 548
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.136 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Procollagen lysine-2-oxoglutarate-5-dioxygenase 3
Alternate Names PLOD3; Procollagen lysine-2-oxoglutarate-5-dioxygenase 3; LH3; Lysyl hydroxylase 3
Uniprot No. O60568
Application The quantitative human PLOD3 (glycosylated) sandwich ELISA kit is designed to measure the concentration of glycosylated PLOD3 in biological samples. This is valuable for research investigating collagen-related disorders, such as fibrosis, skeletal abnormalities, and cancer. The quantitative data obtained from this kit allows for precise analysis of PLOD3 levels, aiding in understanding its role in collagen biosynthesis and disease pathogenesis.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 85-110%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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