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Quantitative Human PRB1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX169)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human PRB1 (glycosylated) sandwich ELISA kit is designed to detect human basic salivary proline rich protein 1 (PRB1) levels. PRB1 is a salivary protein that contributes to the formation of the acquired enamel pellicle, playing a role in protecting teeth from demineralization and influencing calcium binding. The kit is suitable for various biological samples such as biological fluids. Its sensitivity is 0.758 ng/mL, which can accurately detect low concentrations of PRB1 in the sample.
Target PRB1
O-Glycosylation Site 40, 87, 150, 330
Sample Types Biological fluids
Sample Volume 100 μL
Sensitivity 0.758 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 2.5 ng/mL-100 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Basic salivary proline rich protein 1
Alternate Names PRB1; Basic salivary proline rich protein 1; PRB1L; PRPPRB1; Proline-rich protein bstNI subfamily 1
Uniprot No. P04280
Application The quantitative human PRB1 (glycosylated) sandwich ELISA kit is utilized in research investigating oral health, dental caries, and salivary protein composition. Researchers employ this ELISA to quantify PRB1 levels, providing insights into its role in oral homeostasis and potential diagnostic applications related to salivary function and dental health.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-110%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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