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Quantitative Human PRB1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX169

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Specifications
Product Description
The quantitative human PRB1 (glycosylated) sandwich ELISA kit is designed to detect human basic salivary proline rich protein 1 (PRB1) levels. PRB1 is a salivary protein that contributes to the formation of the acquired enamel pellicle, playing a role in protecting teeth from demineralization and influencing calcium binding. The kit is suitable for various biological samples such as biological fluids. Its sensitivity is 0.758 ng/mL, which can accurately detect low concentrations of PRB1 in the sample.
Target
PRB1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Biological fluids
Sample Volume
100 μL
Sensitivity
0.758 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
2.5 ng/mL-100 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Basic salivary proline rich protein 1
Alternate Names
PRB1; Basic salivary proline rich protein 1; PRB1L; PRPPRB1; Proline-rich protein bstNI subfamily 1
Uniprot No.
O-Glycosylation Site
40, 87, 150, 330
Application Notes
Application
The quantitative human PRB1 (glycosylated) sandwich ELISA kit is utilized in research investigating oral health, dental caries, and salivary protein composition. Researchers employ this ELISA to quantify PRB1 levels, providing insights into its role in oral homeostasis and potential diagnostic applications related to salivary function and dental health.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-110%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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