Quantitative Human PROM1 (Glycosylated) Sandwich ELISA Kit

The quantitative human PROM1 (glycosylated) sandwich ELISA kit is designed to detect human prominin 1 (PROM1) levels. PROM1 is a transmembrane glycoprotein expressed on the surface of various stem and progenitor cells, playing a critical role in cell differentiation, proliferation, and maintaining stem cell properties. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.124 ng/mL, which can accurately detect low concentrations of PROM1 in the sample.

PROM1

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.124 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Prominin 1

PROM1; Prominin 1; CD133; Prominin-like protein 1; Antigen AC133

220, 274, 395, 414, 548, 580, 729, 730

The quantitative human PROM1 (glycosylated) sandwich ELISA kit is utilized in research to investigate the expression patterns and regulation of PROM1, particularly in studies related to stem cell biology, cancer research, and regenerative medicine. By accurately quantifying PROM1 levels, researchers can explore its role in disease mechanisms, assess its potential as a biomarker for stem cells or cancer stem cells, and evaluate the effects of therapeutic interventions targeting this protein.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.