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Quantitative Human PROM1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX56)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human PROM1 (glycosylated) sandwich ELISA kit is designed to detect human prominin 1 (PROM1) levels. PROM1 is a transmembrane glycoprotein expressed on the surface of various stem and progenitor cells, playing a critical role in cell differentiation, proliferation, and maintaining stem cell properties. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.124 ng/mL, which can accurately detect low concentrations of PROM1 in the sample.
Target PROM1
N-Glycosylation Site 220, 274, 395, 414, 548, 580, 729, 730
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.124 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Prominin 1
Alternate Names PROM1; Prominin 1; CD133; Prominin-like protein 1; Antigen AC133
Uniprot No. O43490
Application The quantitative human PROM1 (glycosylated) sandwich ELISA kit is utilized in research to investigate the expression patterns and regulation of PROM1, particularly in studies related to stem cell biology, cancer research, and regenerative medicine. By accurately quantifying PROM1 levels, researchers can explore its role in disease mechanisms, assess its potential as a biomarker for stem cells or cancer stem cells, and evaluate the effects of therapeutic interventions targeting this protein.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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