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Quantitative Human PSMα5 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX343

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Specifications
Product Description
The quantitative human PSMα5 (glycosylated) sandwich ELISA kit is designed to detect human proteasome subunit α type 5 (PSMα5) levels. PSMα5 is a component of the 20S proteasome. The proteasome is a protein complex, which is responsible for the degradation of intracellular proteins. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.120 ng/mL, which can accurately detect low concentrations of PSMα5 in the sample.
Target
PSMα5
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.120 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Proteasome subunit α type 5
Alternate Names
PSMα5; Proteasome subunit α type 5; PSC5; Multicatalytic endopeptidase complex zeta chain
Uniprot No.
O-Glycosylation Site
198
Application Notes
Application
The quantitative human PSMα5 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated PSMα5 levels. It is useful for studying the role of PSMα5 in proteasome assembly.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 95-105%;
Plasma sample: n=4, 90-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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