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Quantitative Human RCN3 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX729

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Specifications
Product Description
The quantitative human RCN3 (glycosylated) sandwich ELISA kit is designed to detect human reticulocalbin 3 (RCN3) levels. RCN3 is a calcium-binding protein, which is located in the endoplasmic reticulum. RCN3 plays a role in calcium homeostasis. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.134 ng/mL, which can accurately detect low concentrations of RCN3 in the sample.
Target
RCN3
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.134 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Reticulocalbin 3
Alternate Names
RCN3; Reticulocalbin 3
Uniprot No.
N-Glycosylation Site
140
Application Notes
Application
The quantitative human RCN3 (glycosylated) sandwich ELISA kit is designed to quantitatively measure glycosylated RCN3 in samples. This quantification is important for research on calcium signaling and protein processing. The kit also has applications in investigations of cellular stress.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 85-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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