Quantitative Human TNFSF12 (Glycosylated) Sandwich ELISA Kit

The quantitative human TNFSF12 (glycosylated) sandwich ELISA kit is designed to detect human tumor necrosis factor ligand superfamily, member 12 (TNFSF12) levels. TNFSF12 is a cytokine belonging to the TNF superfamily that plays significant roles in inflammation, angiogenesis, cell proliferation, and apoptosis. It interacts with its receptor Fn14 to regulate various biological processes involved in both normal physiology and pathological conditions. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 7.293 pg/mL, which can accurately detect low concentrations of TNFSF12 in the sample.

TNFSF12

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, serum, plasma

100 μL

7.293 pg/mL

Quantitative sandwich ELISA

20 pg/mL-800 pg/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Tumor necrosis factor ligand superfamily, member 12

TNFSF12; Tumor necrosis factor ligand superfamily, member 12; APO3L; DR3LG; APO3 ligand

139

The quantitative human TNFSF12 (glycosylated) sandwich ELISA kit is utilized in research settings to investigate the expression and regulation of TNFSF12, particularly in studies related to inflammatory diseases, cancer, and autoimmune disorders. By quantifying TNFSF12 levels, researchers can explore its role in disease mechanisms, assess its potential as a biomarker for disease progression, and evaluate the effects of therapeutic interventions targeting this cytokine.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 95-110%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.