Quantitative Human TRAM1 (Glycosylated) Sandwich ELISA Kit

The quantitative human TRAM1 (glycosylated) sandwich ELISA kit is designed to detect human translocation associated membrane protein 1 (TRAM1) levels. TRAM1 is an integral membrane protein located in the endoplasmic reticulum (ER). It plays a role in protein translocation, a process by which newly synthesized proteins are transported across the ER membrane. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.081 ng/mL, which can accurately detect low concentrations of TRAM1 in the sample.

TRAM1

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates

100 μL

0.081 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Translocation associated membrane protein 1

TRAM1; Translocation associated membrane protein 1; TRAM; TRAMP

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The quantitative human TRAM1 (glycosylated) sandwich ELISA kit is designed to determine the concentration of this protein in biological samples. The kit finds application in research related to cell biology, protein synthesis, and ER functions. It is valuable in studies investigating protein translocation mechanisms and ER-related disorders.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-95%;
Plasma sample: n=4, 90-105%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.