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Quantitative Human TRAM1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX605)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human TRAM1 (glycosylated) sandwich ELISA kit is designed to detect human translocation associated membrane protein 1 (TRAM1) levels. TRAM1 is an integral membrane protein located in the endoplasmic reticulum (ER). It plays a role in protein translocation, a process by which newly synthesized proteins are transported across the ER membrane. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.081 ng/mL, which can accurately detect low concentrations of TRAM1 in the sample.
Target TRAM1
N-Glycosylation Site 56
Sample Types Tissue homogenates
Sample Volume 100 μL
Sensitivity 0.081 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Translocation associated membrane protein 1
Alternate Names TRAM1; Translocation associated membrane protein 1; TRAM; TRAMP
Uniprot No. Q15629
Application The quantitative human TRAM1 (glycosylated) sandwich ELISA kit is designed to determine the concentration of this protein in biological samples. The kit finds application in research related to cell biology, protein synthesis, and ER functions. It is valuable in studies investigating protein translocation mechanisms and ER-related disorders.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 90-105%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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