Recombinant Crimean-Congo hemorrhagic fever virus Envelopment polyprotein (GP) (248-519 aa) [MBP/His-Tag], E. coli

Recombinant Crimean-Congo hemorrhagic fever virus Envelopment polyprotein (248-519 aa) was expressed in E. coli with a MBP-tag at the N-terminus and a 6xHis-tag at the C-terminus. The biological activity was determined by its binding ability in a functional ELISA. This product can be used in ELISA, WB, IP.

Crimean-Congo hemorrhagic fever virus

248-519 aa

NLKMEIILTLSQGLKKYYGKILRLLQLTLEEDTEGLLEWCKRNLGLDCDDTFFQKRIEEFFITGEGHFNEVLQFRTPGTLSTTESTPAGLPTAEPFKSYFAKGFLSIDSGYYSAKCYSGTSNSGLQLINITRHSTRIVDTPGPKITNLKTINCINLKASIFKEHREVEINVLLPQVAVNLSNCHVVIKSHVCDYSLDIDGAVRLPHIYHEGVFIPGTYKIVIDKKNKLNDRCTLFTDCVIKGREVRKGQSVLRQYKTEIRIGKASTGSRRLL

MBP-tag at the N-terminus and 6xHis-tag at the C-terminus

74.5 kDa

>90%, determined by SDS-PAGE.

Unconjugated

GP

Envelopment polyprotein

Glycoprotein C and glycoprotein N interact with each other and are present at the surface of the virion. They are able to attach the virion to host cell receptors. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Also promotes fusion of viral membrane with host endosomal membrane after endocytosis of the virion.

M polyprotein; GP