GlycoErase™ AXIN2 Knockout HEK293 Cell Line (CAT#: GLJF-0825-JF387)

Description	GlycoErase™ AXIN2 knockout HEK293 cell line is a human embryonic kidney (HEK293) model in which AXIN2 expression has been disrupted. AXIN2 regulates β-catenin stability in the Wnt signaling pathway, and its dysregulation is associated with colorectal and other cancers. The knockout of AXIN2 in the HEK293 background enables precise studies of Wnt/β-catenin signaling dynamics, transcriptional regulation, and cancer biology, while supporting applications in oncology research, developmental biology, and Wnt-targeted drug discovery.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	AXIN2
Full Name	Axin 2
Alternative Name	AXIL; ODCRCS
Location	17q24.1
Gene ID	8313
Summary	AXIN2 encodes a regulator of beta-catenin stability in the Wnt pathway. Similar to rodent homologs, it forms complexes with APC, beta-catenin, and GSK-3β to promote beta-catenin degradation. Genetic alterations are associated with colorectal cancer and other malignancies.