GlycoErase™ HEK293F-ΔSLC35D1 Cell Line

This SLC35D1 knockout (HEK293F) cell line lacks the SLC35D1 protein, a solute carrier family 35 member D1. Glycosylation of cellular glycoconjugates occurs in the endoplasmic reticulum (ER) and Golgi compartment, and requires transport of nucleotide sugars from the cytosol into the lumen of the ER and Golgi by specific transporters. The protein encoded by this gene resides in the ER, and transports both UDP-glucuronic acid (UDP-GlcA) and UDP-N-acetylgalactosamine (UDP-GalNAc) from the cytoplasm to the ER lumen. Loss of SLC35D1 function may disrupt nucleotide sugar transport.

KO Cell Lines

Human

Epithelial-like, adherent

1:2~1:4

HEK293F

Embryonic kidney

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

DMEM

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

SLC35D1

Solute carrier family 35 member D1

KIAA0260, UGTREL7

1p31.3

Transports UDP-glucuronic acid and UDP-N-acetylgalactosamine from the cytoplasm to the endoplasmic reticulum lumen, essential for glycosylation of cellular glycoconjugates.