GlycoErase™ ST3GAL1 Knockout HepG2 Cell Line (CAT#: GLJF-0425-JF1110)

Description	ST3GAL1 knockout HepG2 cell line lacks ST3GAL1 protein, ST3 beta-galactoside alpha-2,3-sialyltransferase 1, catalyzing sialylation in the Golgi of liver cells. ST3GAL1 knockout in HepG2 cells allows investigation of sialylation disruption in liver cancer, potentially affecting alpha-2,3-sialylation of glycans on hepatocytes, cell surface sialylation of liver cells, and sialic acid dependent liver tumor properties.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Functional assay
Size	2 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	ST3GAL1
Full Name	ST3 Beta-galactoside alpha-2,3-sialyltransferase 1
Alternative Name	ST3GalA.1, ST3O, SIAT4A, SIATFL
Location	8q24.22
Gene ID	6482
Summary	Catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates; primarily located in the Golgi but can be processed into a soluble form.