GlycoFlux™ Human LDHB Overexpression HepG2 Cell Line

GlycoFlux™ human LDHB overexpression HepG2 cell line is a stable cell line designed to express lactate dehydrogenase B (LDHB), which catalyzes the reversible interconversion of pyruvate and lactate with NADH/NAD⁺. LDHB is widely expressed and includes a unique isoform generated by stop codon readthrough, targeted to peroxisomes. Mutations cause LDHB deficiency, linked to metabolic disorders. This model provides a system to study glycolysis, lactate shuttling, and metabolic disease mechanisms.

Overexpression Cell Lines

Human

Epithelial-like, adherent

1:4~1:6

HepG2

Hepatoblastoma

Liver

Hepatoblastoma

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

qPCR, Sanger Sequencing

DMEM & FBS & Glutamine

Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

LDHB

Lactate Dehydrogenase B

LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281

12p12.1

This gene encodes the B chain of lactate dehydrogenase, responsible for the reversible interconversion of pyruvate and lactate, alongside NADH and NAD+, following glycolysis. Multiple transcript variants exist, including a C-terminally extended isoform generated via stop codon readthrough, which localizes to peroxisomes. Mutations cause lactate dehydrogenase B deficiency. Pseudogenes are present on chromosomes X, 5, and 13.