GlycoFlux™ Human POMC Overexpression HEK293 Cell Line

GlycoFlux™ human POMC overexpression HEK293 cell line is a stable glycoengineered line that constitutively overexpresses proopiomelanocortin (POMC), a secretory preprohormone cleaved by prohormone convertases into ACTH, β-lipotropin, and additional bioactive peptides in a tissue-dependent manner. This model enables controlled interrogation of peptide processing, secretory-pathway capacity, and how glycosylation-dependent sorting/trafficking steps (e.g., ER-Golgi transit, cargo receptor engagement) shape peptide stability and yield, supporting formulation and media screens for optimized hormone bioproduction.

Overexpression Cell Lines

Human

Epithelial-like, adherent

1:2~1:4

HEK293

Embryonic kidney

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

qPCR, Sanger Sequencing

FBS & Penicillin/Streptomycin & Proline & DMEM

Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

POMC

Proopiomelanocortin

LPH; MSH; NPP; POC; ACTH; CLIP; OBAIRH

2p23.3

This gene encodes a preproprotein processed in a tissue-specific manner by prohormone convertases, producing up to ten active peptides. In the pituitary, major products include adrenocorticotrophin and beta-lipotropin, while in other tissues, peptides regulate pain, pigmentation, immunity, and energy balance. Mutations are linked to early-onset obesity, adrenal insufficiency, and red hair pigmentation.