GlycoFlux™ Human PPP1R3A Overexpression Glycoengineered Cell (CAT#: GLJF-0925-JF57)

Description	GlycoFlux™ human PPP1R3A overexpression glycoengineered cell is a stable cell line elevating the glycogen-tethering PP1 regulator (GM) to enhance PP1 targeting to glycogen and dephosphorylation of glycogen synthase/phosphorylase. It enables rapid glycogen loading studies, UDP-glucose partitioning vs Golgi galactosylation/sialylation trade-offs, and time-course mapping of secreted glycoprotein heterogeneity.
Product Type	Overexpression Cell Lines
Cell Line	As requested by the client.

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PPP1R3A
Full Name	Protein Phosphatase 1 Regulatory Subunit 3A
Alternative Name	GM; PP1G; PPP1R3
Location	7q31.1
Gene ID	5506
Summary	The glycogen-associated form of protein phosphatase-1 (PP1) derived from skeletal muscle is a heterodimer composed of a 37-kD catalytic subunit and a 124-kD targeting and regulatory subunit. This gene encodes the regulatory subunit which binds to muscle glycogen with high affinity, thereby enhancing dephosphorylation of glycogen-bound substrates for PP1 such as glycogen synthase and glycogen phosphorylase kinase.