GlycoFlux™ Human PPP1R3D Overexpression Raji Cell Line

GlycoFlux™ human PPP1R3D overexpression Raji cell line is a stable cell line engineered to overexpress PPP1R3D, a glycogen-targeting PP1 regulatory subunit that dictates phosphatase localization and substrate specificity in the balance between kinase and phosphatase signaling. This system enables investigation of PP1 compartmentalization, glycogen-coupled phosphorylation cycles, and metabolic signal integration.

Overexpression Cell Lines

Human

Lymphoblast-like, suspension growth

1:2~1:4

Raji

Burkitt's lymphoma

B lymphocyte (B cell)

Mature B cell, widely used in EBV-related studies

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

qPCR, Sanger Sequencing

FBS & RPMI

Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

PPP1R3D

Protein Phosphatase 1 Regulatory Subunit 3D

PPP1R6

20q13.33

Phosphorylation of serine and threonine residues in proteins is a crucial step in the regulation of many cellular functions ranging from hormonal regulation to cell division and even short-term memory. The level of phosphorylation is controlled by the opposing actions of protein kinases and protein phosphatases. Protein phosphatase 1 (PP1) is 1 of 4 major serine/threonine-specific protein phosphatases which have been identified in eukaryotic cells. PP1 associates with various regulatory subunits that dictate its subcellular localization and modulate its substrate specificity. Several subunits that target PP1 to glycogen have been identified. This gene encodes a glycogen-targeting subunit of PP1.