GlycoErase™ PPP1R3D Knockout Jurkat Cell Line (CAT#: GLJF-0825-JF807)

Description	GlycoErase™ PPP1R3D knockout Jurkat cell line is a Jurkat model deficient in PPP1R3D, a glycogen-targeting subunit of PP1. This knockout is ideal for exploring glycogen regulation, kinase-phosphatase signaling balance, and short-term metabolic regulation.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Suspension-growing
Passage Ratio	1:3~1:5
Cell Line	Jurkat cell line
Primary Disease	T-cell acute lymphoblastic leukemia (T-ALL)
Lineage	T Lymphocyte
Lineage Subtype	T-cell acute lymphoblastic leukemia (T-ALL)

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PPP1R3D
Full Name	Protein Phosphatase 1 Regulatory Subunit 3D
Alternative Name	PPP1R6
Location	20q13.33
Gene ID	5509
Summary	Phosphorylation of serine and threonine residues in proteins is a crucial step in the regulation of many cellular functions ranging from hormonal regulation to cell division and even short-term memory. The level of phosphorylation is controlled by the opposing actions of protein kinases and protein phosphatases. Protein phosphatase 1 (PP1) is 1 of 4 major serine/threonine-specific protein phosphatases which have been identified in eukaryotic cells. PP1 associates with various regulatory subunits that dictate its subcellular localization and modulate its substrate specificity. Several subunits that target PP1 to glycogen have been identified. This gene encodes a glycogen-targeting subunit of PP1.