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GlycoFlux™ MMLV-Human ACLY Viral Particle (CAT#: GLJF-1025-JF399)

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SPECIFIC INQUIRY
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Description GlycoFlux™ MMLV-ACLY Viral Particle delivers ACLY (ATP-citrate lyase), generating cytosolic acetyl-CoA from citrate to fuel lipogenesis and cholesterogenesis; the vector supports stable line construction for metabolic rewiring, lipid synthesis control, and acetyl-CoA flux assays.
Product Type Recombinant MMLV Retrovirus
Target ACLY
Species Human
Promotor MMLV 5' LTR promoter
Packaging System Retrovirus
Packaging Cell HEK293T
Application This MMLV vector is a common retroviral vector system that permanently integrates a gene into the host cell's genome, allowing for long-term, sustained expression. It is a popular tool for gene manipulation in dividing cells and for generating iPS cells in biomedical research.
Shipping Shipped on dry ice with temperature held at or below -80°C.
Storage Store at -80°C to maintain their titer and stability.
Handling Notes Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.
Product Disclaimer These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Titer Assay qPCR
Mycoplasma Test Negative
Sterility Sterility testing confirmed no microbial contamination.
Transduction Evaluation On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation Creative Biolabs verifies all retroviral vectors by a PCR-based proviral integration check. In brief, cells are transduced with serial dilutions of the retroviral preparation and, after several days, genomic DNA is extracted. A predefined segment of the expected retroviral insert is then amplified by PCR to confirm correct identity and integration.
Full Name Atp Citrate Lyase
Alternative Name ACL; ATPCL; CLATP
Location 17q21.2
Gene ID 47
Summary ATP citrate lyase is an enzyme that generates cytosolic acetyl-CoA from citrate and CoA in many tissues. It operates as a tetramer and catalyzes the conversion of citrate to acetyl-CoA and oxaloacetate, with ATP hydrolysis. Acetyl-CoA plays a role in lipogenesis, cholesterogenesis, and possibly acetylcholine biosynthesis in the nervous system.
For Research Use Only.
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