Description

GlycoFlux™ MMLV-MGAT1 Viral Particle provides MGAT1, the medial-Golgi GlcNAc-transferase required to initiate hybrid/complex N-glycan formation; the MMLV system establishes stable mammalian expression lines for branching control, OST occupancy studies, and rescue of MGAT1 loss.

Product Type

Recombinant MMLV Retrovirus

Target

MGAT1

Species

Human

Promotor

MMLV 5' LTR promoter

Packaging System

Retrovirus

Packaging Cell

HEK293T

Application

This MMLV vector is a common retroviral vector system that permanently integrates a gene into the host cell's genome, allowing for long-term, sustained expression. It is a popular tool for gene manipulation in dividing cells and for generating iPS cells in biomedical research.

Shipping

Shipped on dry ice with temperature held at or below -80°C.

Storage

Store at -80°C to maintain their titer and stability.

Handling Notes

Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.

Product Disclaimer

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

Titer Assay	qPCR
Mycoplasma Test	Negative
Sterility	Sterility testing confirmed no microbial contamination.
Transduction Evaluation	On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation	Creative Biolabs verifies all retroviral vectors by a PCR-based proviral integration check. In brief, cells are transduced with serial dilutions of the retroviral preparation and, after several days, genomic DNA is extracted. A predefined segment of the expected retroviral insert is then amplified by PCR to confirm correct identity and integration.

Full Name	Alpha-1,3-mannosyl-glycoprotein 2-Beta-O-acetylglucosaminyltransferase
Alternative Name	MGAT; GnTI; GLCNAC-TI; GLYT1; GNT-1
Location	5q35.3
Gene ID	4245
Summary	Medial-Golgi enzyme needed for producing hybrid and complex N-glycans, enhancing structural diversity.

GlycoFlux™ MMLV-Human MGAT1 Viral Particle (CAT#: GLJF-1025-JF335)

Specifications

Quality Control

Target Information

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