Specifications Quality Control Target Information Related Products

Specifications

Description

GlycoFlux™ MMLV-PDK3 Viral Particle provides PDK3, a potent PDC E1α kinase that diverts pyruvate away from the TCA cycle; configured to build durable, stably transduced mammalian cells, enabling mitochondrial control studies, hypoxia responses, and drug-response profiling.

Product Type

Recombinant MMLV Retrovirus

Target

PDK3

Species

Human

Promotor

MMLV 5' LTR promoter

Packaging System

Retrovirus

Packaging Cell

HEK293T

Application

This MMLV vector is a common retroviral vector system that permanently integrates a gene into the host cell's genome, allowing for long-term, sustained expression. It is a popular tool for gene manipulation in dividing cells and for generating iPS cells in biomedical research.

Shipping

Shipped on dry ice with temperature held at or below -80°C.

Storage

Store at -80°C to maintain their titer and stability.

Handling Notes

Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.

Product Disclaimer

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

Quality Control

Titer Assay

qPCR

Mycoplasma Test

Negative

Sterility

Sterility testing confirmed no microbial contamination.

Transduction Evaluation

On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.

Insert Identity Confirmation

Creative Biolabs verifies all retroviral vectors by a PCR-based proviral integration check. In brief, cells are transduced with serial dilutions of the retroviral preparation and, after several days, genomic DNA is extracted. A predefined segment of the expected retroviral insert is then amplified by PCR to confirm correct identity and integration.

Target Information

Full Name

Pyruvate Dehydrogenase Kinase 3

Alternative Name

CMTX6; GS1-358P8.4

Location

Xp22.11

Gene ID

5165

Summary

The pyruvate dehydrogenase complex converts pyruvate to acetyl-CoA, linking glycolysis to the TCA cycle. This gene encodes one of the pyruvate dehydrogenase kinases that inhibit the complex by phosphorylating the E1 alpha subunit.

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