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GlycoFlux™ MMLV-Human PPP1R13B Viral Particle (CAT#: GLJF-1025-JF472)

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Description GlycoFlux™ MMLV-PPP1R13B Viral Particle encodes PPP1R13B (ASPP1), a p53-pathway coactivator with ankyrin repeats/SH3 that enhances binding to pro-apoptotic targets under E2F control; the system establishes stable mammalian models to dissect p53 transcriptional thresholds and stress responses.
Product Type Recombinant MMLV Retrovirus
Target PPP1R13B
Species Human
Promotor MMLV 5' LTR promoter
Packaging System Retrovirus
Packaging Cell HEK293T
Application This MMLV vector is a common retroviral vector system that permanently integrates a gene into the host cell's genome, allowing for long-term, sustained expression. It is a popular tool for gene manipulation in dividing cells and for generating iPS cells in biomedical research.
Shipping Shipped on dry ice with temperature held at or below -80°C.
Storage Store at -80°C to maintain their titer and stability.
Handling Notes Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.
Product Disclaimer These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Titer Assay qPCR
Mycoplasma Test Negative
Sterility Sterility testing confirmed no microbial contamination.
Transduction Evaluation On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation Creative Biolabs verifies all retroviral vectors by a PCR-based proviral integration check. In brief, cells are transduced with serial dilutions of the retroviral preparation and, after several days, genomic DNA is extracted. A predefined segment of the expected retroviral insert is then amplified by PCR to confirm correct identity and integration.
Full Name Protein Phosphatase 1 Regulatory Subunit 13B
Alternative Name p85; ASPP1; p53BP2-like
Location 14q32.33
Gene ID 23368
Summary The encoded ASPP family protein interacts with p53 family members to enhance DNA binding and transcription of proapoptotic targets. It contains ankyrin repeats and an SH3 domain and is regulated by E2F transcription factors.
For Research Use Only.
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