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GlycoFlux™ MMLV-Human PPP1R3A Viral Particle (CAT#: GLJF-1025-JF481)

Datasheet

SPECIFIC INQUIRY
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Description GlycoFlux™ MMLV-PPP1R3A Viral Particle delivers PPP1R3A, the skeletal-muscle glycogen-targeting subunit that steers PP1 to glycogen synthase and phosphorylase kinase; the platform establishes stable lines for glycogen synthesis activation and flux mapping.
Product Type Recombinant MMLV Retrovirus
Target PPP1R3A
Species Human
Promotor MMLV 5' LTR promoter
Packaging System Retrovirus
Packaging Cell HEK293T
Application This MMLV vector is a common retroviral vector system that permanently integrates a gene into the host cell's genome, allowing for long-term, sustained expression. It is a popular tool for gene manipulation in dividing cells and for generating iPS cells in biomedical research.
Shipping Shipped on dry ice with temperature held at or below -80°C.
Storage Store at -80°C to maintain their titer and stability.
Handling Notes Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.
Product Disclaimer These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Titer Assay qPCR
Mycoplasma Test Negative
Sterility Sterility testing confirmed no microbial contamination.
Transduction Evaluation On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation Creative Biolabs verifies all retroviral vectors by a PCR-based proviral integration check. In brief, cells are transduced with serial dilutions of the retroviral preparation and, after several days, genomic DNA is extracted. A predefined segment of the expected retroviral insert is then amplified by PCR to confirm correct identity and integration.
Full Name Protein Phosphatase 1 Regulatory Subunit 3A
Alternative Name GM; PP1G; PPP1R3
Location 7q31.1
Gene ID 5506
Summary The skeletal muscle form of glycogen-associated PP1 consists of a catalytic subunit and a targeting/regulatory subunit. This gene encodes the regulatory component that binds glycogen and directs PP1 toward substrates like glycogen synthase and phosphorylase kinase.
For Research Use Only.
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