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| Description | GlycoFlux™ MMLV-PRKACA Viral Particle delivers PRKACA, a catalytic subunit of PKA released upon cAMP activation; this tool establishes stable mammalian expression lines for cAMP axis mapping, substrate panels, and disease-variant analysis. |
| Product Type | Recombinant MMLV Retrovirus |
| Target | PRKACA |
| Species | Human |
| Promotor | MMLV 5' LTR promoter |
| Packaging System | Retrovirus |
| Packaging Cell | HEK293T |
| Application | This MMLV vector is a common retroviral vector system that permanently integrates a gene into the host cell's genome, allowing for long-term, sustained expression. It is a popular tool for gene manipulation in dividing cells and for generating iPS cells in biomedical research. |
| Shipping | Shipped on dry ice with temperature held at or below -80°C. |
| Storage | Store at -80°C to maintain their titer and stability. |
| Handling Notes | Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection. |
| Product Disclaimer | These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application. |
| Titer Assay | qPCR |
| Mycoplasma Test | Negative |
| Sterility | Sterility testing confirmed no microbial contamination. |
| Transduction Evaluation | On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function. |
| Insert Identity Confirmation | Creative Biolabs verifies all retroviral vectors by a PCR-based proviral integration check. In brief, cells are transduced with serial dilutions of the retroviral preparation and, after several days, genomic DNA is extracted. A predefined segment of the expected retroviral insert is then amplified by PCR to confirm correct identity and integration. |
| Full Name | Protein Kinase Camp-Activated Catalytic Subunit Alpha |
| Alternative Name | CAFD1; PKACA; PPNAD4 |
| Location | 19p13.12 |
| Gene ID | 5566 |
| Summary | This gene encodes a catalytic subunit of protein kinase A (PKA), which becomes active when cAMP binds the regulatory subunits, releasing the catalytic monomers. Mutations or duplications involving this gene are linked to adrenal hyperplasia and Cushing's syndrome. |