Specifications Quality Control Target Information Related Products

Specifications

Description

GlycoFlux™ MSCV-PDK4 Viral Particle establishes stable PDK4 expression by MSCV integration, enabling hormone-responsive control of PDH phosphorylation and fasting-mimetic fuel switching. Readouts include PDH activity, RER (fuel preference) in vitro surrogates, and lipid oxidation markers.

Product Type

Recombinant MSCV Retrovirus

Target

PDK4

Species

Human

Promotor

MSCV 5' LTR promoter

Packaging System

Retrovirus

Packaging Cell

HEK293T

Application

This MSCV vector is a common retroviral vector system used to achieve high-efficiency, stable gene delivery into target cells, such as hematopoietic stem cells. It ensures long-term, sustained expression by stably integrating the gene into the host cell's genome.

Shipping

Dispatched on dry ice with temperature held at or below -80°C.

Storage

Store at -80°C to preserve titer and stability.

Handling Notes

Store at -80°C and avoid repeated freeze-thaw cycles to preserve viral titer. For safety, handle materials in a biological safety cabinet while wearing appropriate PPE (lab coat, gloves, and eye protection).

Product Disclaimer

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

Quality Control

Titer Assay

qPCR

Mycoplasma Test

Negative

Sterility

Sterility testing confirmed no microbial contamination.

Transduction Evaluation

Creative Biolabs offers, upon request, in vitro and in vivo transduction assays to verify lentiviral targeting and to quantify transgene expression and functional output.

Insert Identity Confirmation

All Creative Biolabs retroviral vectors undergo PCR-based verification of proviral integration. Cells are transduced across a dilution series of the retroviral preparation; after several days, genomic DNA is isolated, and a defined region of the expected insert is PCR-amplified to confirm correct identity and integration.

Target Information

Full Name

Pyruvate Dehydrogenase Kinase 4

Location

7q21.3

Gene ID

5166

Summary

This mitochondrial protein, part of the PDK/BCKDK kinase family, inhibits the pyruvate dehydrogenase complex by phosphorylation. Located in the mitochondrial matrix, its expression is regulated by glucocorticoids, retinoic acid, and insulin.

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GlycoFlux™ MSCV-Human PDK4 Viral Particle